This is a photo Dr. Frank Shallenberger using a Longevity EXT-120 ozone unit made in Canada  to fill a hypodermic syringe with the proper concentration of ozone gas and  oxygen.  Longevity was also the brand of ozone generator Dr. Rowen and Robbins took to Sierra Leone to treat Ebola. I own a used Longevity EX-120 and it is a very robustly constructed unit.
    A word of warning before you read on. This page is not written to encourage the average Joe or Johanna to go out and do ozone DIV.  It should only be attempted by those that are intimately familiar with the health sciences and have common sense skills when experimenting with innovative procedures. To those veterinarians, other health professionals, owners of livestock with dire infectious conditions that are seeking new paradigms, ozone DIV is well worth studying as I have found. In the end, every organism is unique as is its reaction to external stimuli. There are never any guarantees in life, but one can often increase the odds in your favor.

     My initial journey into oxidative medicine begin a number of years ago with the purchase and reading of The Oxygen Prescription: The Miracle of Oxidative Therapies by Nathaniel Altman. At the time, I was primarily interested in Hydrogen Peroxide therapy and fascinated by the clinical work of William Campbell Douglass II, MD in that field. After studying the Altman book, along with doing more research from the recognized ozone groups around the world, I found ozone DIV therapy to be widely considered a dangerous procedure which stimulated me to shelf any interest in it in favor of more work with hydrogen peroxide. However, over the intervening years ozone replaced my hydrogen peroxide interests with my discovery of the work of the ozone gurus in the USA, Robert Rowen MD and Howard Robins MD, I became far more curious and receptive to ozone DIV as a cheap "miracle" drug by this simple DIV route. I collected more knowledge along with appropriate instrumentation, just in case,  if I should ever want to actually experiment with ozone. For the most part, I felt if something dire infected me or my animals, I could easily use it, but for the time being, I would just set on this knowledge.  That all changed with the 2018 flu. I not only was infected with this virus, but it settled in my lungs and didn't seem to want to leave. After about four weeks of not much improvement while administering my herbal nebulizer solutions for my lungs to limited success, I broke my reluctance for ozone and decided I would try ozone DIV on myself. After all, the proverb: "Physician, heal thyself" has long been my mantra in medicinal matters--who better to first attempt it than myself on myself? The trigger was when I suffered a relapse of my viral lung involvement after about 3 weeks on a Friday night accompanied with another low grade fever episode. After viewing the below YouTube videos a few months previously, I decided the next day would be my first ozone DIV treatment! The first video seemed to show how effective ozone can be against the flu and the second Domb video demonstrated how simplistic it was even for self-administration. The result is my work with ozone DIV as depicted in the top photo of my infusion of ozone via a syringe pump into myself. This webpage will be a study of what I discovered and should be of benefit to others in treating man or beast for really tough infections.
     Ozone IV protocols as practiced by the majority of medical personnel the world around are primarily limited to the traditional  two: Major Autohemotherapy (MAH) and Minor Autohemotherapy procedures. These two are the commonly approved methods in Europe and most other parts of the world.  MAH involves withdrawing approximately 250cc of the patient's blood into an IV bag, adding ozone/oxygen gas mixture with a syringe, gently mixing and then reinjecting back into the patient. Minor autohemotherapy involves smaller volumes, 2-5 cc of blood drawn into a sterile 30 cc syringe where it is mixed with an already present 10 cc of an ozone/oxygen gas mixture in that syringe, shaken and then slowly reinjected.  Additionally there is currently a relatively new protocol pioneered by Dr. Lahodny of Austria, called the "Ten Pass" or Hyperbaric MultiPass Auto Hemo Ozone Therapy (SOT-hmahot)  which just came onto the scene and offers some exciting results superior to both MAH and minor. Both Dr. Rowen and Dr. Robins are enthusiastic proponents of this method. It does seem like the preferred ozone delivery method, presuming one can find a qualified trained practitioner owning the expensive specialized ozone hyperbaric equipment needed which may be more of a challenge than not. However, it should be of interest here that when Rowen and Robins went to Africa to treat Ebola, they used the DIV method in those primitive conditions. Simplicity does have its charms and advantages with this being particularly true in a veterinary, barn setting! Remember that! Basically, the Hyperbaric multipass method involves the removal of the patient's blood as in MAH, but this time larger volumes of the ozone/oxygen gas is forced under pressure into that removed blood volume resulting in a higher concentration of gas to be dissolved in the blood when compared to the simple mixing without pressure of MAH. Like MAH, this mixture is reinjected but unlike MAH, in multple-passes or multiple treated volumes up to 10 or more times at one setting. Lastly, we have Ozone Direct Intravenous (DIV) that many consider so dangerous.

     DIV ozone was probably one of the very first IV procedures employed in the historic use of medical ozone. Exactly why it has fallen into such ill repute is a bit hard to understand. As Dr. Robins writes, there are no first hand accounts any where on the Internet or medical literature that can document even one legitimate lethal case resulting directly from ozone DIV, just a lot of innuendo. The Italian ozone MD and author, Dr. Bocci is equally critical of ozone DIV methods, often suggesting that the well publicized deaths of two Italian women in his country as an example of its lethality which I might stress again, on closer examination had nothing to do with direct intravenous administration at all! Those deaths were ozone injections into fatty tissue at beauty salons, probably by non-medical personnel, plus these ladies seemed to have other under-laying health conditions.

    Bocci and many others also condemn ozone DIV because they think it is likely to cause an embolism in the bloodstream resulting in possible heart attack or stroke, death. Death by air embolism is one of the most cleaver fictionally used murder procedures on TV/movies alike that has fostered this myth of the lethality of injected air in the vein. It is way, way overrated both in fiction and even in a clinical medical setting! A good relevant quote here was written by Paracelsus:
"What is there that is not poison? All things are poison and nothing is without poison. Solely the dose determines that a thing is not a poison."  This holds very true for an ozone/oxygen gas mixture as well! There are a few important points with Ozone DIV that makes a huge difference from the act of simply injecting mixed atmospheric air into the blood stream. One: ozone/oxygen is being injected into the venous blood system which contains blood depleted of oxygen and accordingly is very oxygen hungry. Injected oxygen/ozone is quickly absorbed on contact, just as it would be if it were in the lung tissue. Two: ozone DIV only uses USP grade pure oxygen that has ozone generated into it and does not contain nitrogen.  Nitrogen is 78% of our atmospheric air. Injected nitrogen is very chemically unreactive!  Nitrogen acts very differently than the highly reactive ozone or oxygen gases in our blood stream by remaining in gas form which allows it to more likely result in an emboli. The fictionalized murder syringes of air are always drawn from the atmosphere, high in nitrogen.  Nevertheless, even very reactive gases like oxygen and ozone does have its absorptive limits in the blood stream. Even oxygen hungry blood can only react with so much oxygen/ozone. Proper infusion rates are important in ozone DIV. That is why I find a mechanical syringe pump very helpful like the one pictured in the above top photo. Dr. Robins and others disagree though they do not elaborate why.  Dr. Robins writes: "In my opinion, DIV should never be performed by pump, only by syringe in the hand of the  therapist."   I can only speculate here for the time being of his reasons. One would never want to rely too heavily on a pump to the detriment of observing all factors during DIV. Perhaps, he feels it would bring on a lackadaisical attitude of the staff or, maybe it is the FDA reports on injuries/deaths that occurred from the use of infusion pumps? It appears most of these FDA reports are for the modern hospital computerized versions where they list software malfunction, alarm errors, user programing issues (button bounce, etc) as the major issues. That is why I prefer the old analog models that do not rely on computer chips, are easy to use, have simple on-and-off switches, no screens, and consist of very limited ways one can foul up with these old types of technology.  What is also perplexing is that the new Ten Pass hyperbaric ozone generators of which Dr. Robins employs all contain self-enclosed syringe vacuum and injection pumps that are automatic. So, why would an automatic syringe pump be ok in one and not the other procedure?  Ok, ok, one can over-infuse blood with little serious consequence, maybe not so much a gas? I grant you that point.  I for one find the infusion pump to be simply a tool that few humans can match in uniform slow delivery versus human hand depression of the syringe's plunger. There is no reason why both the pump and the patient cannot be constantly monitored at the same time with the pump instantly being able to be cut-off or reduced according to patient response. In this instance, old analog technology is to be desired! I will discuss this topic further, later.

     Another fault often levied at ozone DIV is that it can induce bronchospasm - pain, tightness, constriction of the chest, and coughing.  Patients with a history of asthma, COPD, etc are often said to fare worse than others during DIV with an increased chance of acute respiratory failure; however, Dr. Robins disagrees and claims he has had excellent success treating such patients.   In the second YouTube video above, showing Dr. Bill Domb self-administering DIV, he describes some of the sensations that he has experienced during the procedure as tightness in the chest, the feeling that one would cough if breathing too deeply, that he can feel and "hear" the bubbles going through his shoulder, that he can feel them in his heart sometimes.  Notice after he pulls the needle in this video, he does cough once before filming is stopped.   In my experience, I have never felt any such sensations, though I will have a greater tendency to cough within 5-10 minutes after the needle is removed finishing the infusion. I suspect that most of these sensations are in response to too rapid injection times. Using my Harvard syringe pump set at 3.1 ml per minute, I had absolutely no sensations in my arm, shoulder or chest. Dr. Robins advises rates at 1cc per 5-15 seconds. My pump rate is more conservative than that, but I say, hey, what's the hurry? Dr. Robin's first response to bronchospasm sensations in the patient is to slow down the infusion rate. Dr. Robins writes
: "Bronchospasm occurs in many patients, though some patients never experience it at all.  It is very important  to note that this occurs most commonly on the first  treatment and diminishes on subsequent treatments, even though increasing amounts of oxygen/ozone are administrated. In fact, clinical experience has shown that by the 8th through the 12th treatment in virtually all patients, each receiving approximately 55cc at 55mcg/cc have it diminishes to the point that a second syringe may be given (in 10cc increments) with no increased lung irritation."  His theory on why this may happen: "I believe that the ozone and its analogs are reacting with harmful  free radical gases such as carbon monoxide (as much as 3% or more of the gases found in blood), creating larger  than normal amounts of carbon dioxide and other gases that can be eliminated from the blood through respiration. The buildup of these gases around the lung tissue causes the feeling of pressure, tightness or discomfort in the upper chest. This also causes the lungs to try and expel these gases through coughing."

   Dr. Robins practice has done approximately 45,000 patients using MAH, but he quickly found ozone DIV to be more effective. He agrees with the Italian Dr. Bocci: "MAH helps improve most medical diseases and conditions, but rarely cures them." Compare this to Dr. Robin's clinical history of over 18 years of 160,000+ ozone DIV procedures in which he says he has significantly improved and more often completely eliminated the following diseases:

Herpes  I,  II,  VI;  Herpes  zoster (Shingles) including post-herpetic nerve pain;  Epstein-Barr Virus; Cytomeglavirus;  Adenovirus;  Coxsacchie  virus;  Lyme  Disease (all  stages); AIDS;  HIV;  Multiple Sclerosis;  Pulmonary  Fibrosis;  all viral, fungal and bacterial lung infections; all causes of sinusitis;  Viral  meningitis; Measles;  perforated ear  drum;  Rheumatoid  Arthritis;  Lupus;  Scleroderma;  Candidiasis;  E.  coli;  H.  pylori;  Crohn's  disease;  ALS;  RSD/CRPS  (first  in  the  world);  Chronic fatigue Syndrome;  Diabetic gangrenous ulcers; Diabetic peripheral neuropathy; Fungus infections of all types in skin and nails; Hepatitis B, C;  dry eye;  temporary blindness caused by embolism from "mini-stroke";  glaucoma;  HPV;  upper-respiratory  tract  infections (common cold);  all  forms  of  Influenza;  Thrush;  sleeping  disorders;  depression and anxiety (when due to medical  problems; Bells  Palsy; Fibromyalgia; Fibroids  on  uterus  and  thyroid  gland;  decubitus ulcers; infected wounds and ulcers.

     The safety record of Ozone DIV under Dr. Robins is also impressive. He writes that he has only experienced three patients that suffered adverse reactions  in the 160,000+ treatments. Those three involved the appearance of temporary rashes 30 minutes after treatment.

     The 2018 flu was a tough one this year and I can attest to that in person. The CDC writes that hospitalization rates this season have been record-breaking, exceeding end-of-season hospitalization rates for 2014-2015, a high severity for this 2018 H3N2-predominant season. I first felt symptoms of sinus & throat congestion on March 16th, followed three days later by a low grade fever, heavy sensation of the limbs, aching jaw the result of worsening sinusitis with ever thickening yellow mucus formation and discharge. I experienced rare sinus head aches. Lung involvement soon became very pronounced with shallow breathing, coughing, chest pressure, lung "rattling". During this period as an herbalist, I tried a number of my favorite herbal remedies, mostly administered as nebulizer solutions. They seemed to do little, but after about 10 days, I seemed to be on the improve with decreasing lung congestion and cough. Then on April 13th, my initial flu symptoms seemed to reoccur with a episode of another low grade fever, heavy arm sensations and increased lung involvement and coughing worse than ever.   I thought to myself,  enough is enough and tried ozone DIV the next day. Following Dr. Robins protocol, my initial ozone/oxygen volume was 20cc, but at a bit weaker ozone concentration of 49 mcg.  I felt relief that night and the next day for the first time. Two days later, I did another infusion at 34cc at the same concentration of 49mcg of ozone. The next morning, I could finally breath deeply without eliciting a cough and no more lung rattling sensations. I did a third infusion, three days later of 43cc but this time at the suggested higher concentration of 55 mcg. My lungs were feeling better and better. I decided to give another infusion the next day at the same volume and concentration plus the following day for a total of five IV infusions over that week. My lungs finally felt pretty much back to normal and for the first time in this very long episode of the flu, I had achieved a consistent lessening of symptoms and progress that week when I finally started ozone DIV therapy. I was impressed!

     I suspect in retrospect that had I initiated ozone DIV on March 16th when symptoms first appeared, it would have quickly aided my immune system in stopping the H3N2 virus in its tracks similar to how it was described at the beginning of this page in the first YouTube video. I started ozone almost 4 weeks after that first symptoms and it is probably no wonder that it took 5 infusions over about 7 days to regain normalcy.  If one studies the ebola ozone data,  ebola cases were treated within the first few days of symptoms resulting in complete cures. Immediate treatment with ozone is probably an optimum strategy for the most efficacious recovery which would hold true for all infections as well. I will know better next time!

      What is the theory behind the medicinal value of Ozone DIV in treating infections? Certainly, ozone is lethal when in direct contact of virus, bacteria, and fungi, but inside the body, these pathogens are far flung and protected, plus ozone dissipates in micro-seconds in blood.  Dr. Shallenberger probably has the best explanation on the essence of Ozone therapy. He labels ozone not a drug, but a Biological Response Modifier.  It is an immunomodulator that affects different patients, uniquely and appropriately for their individual system needs.  Ozone in part may work on Infectious diseases by activating ancillary mechanisms. Dr. Rowen writes:

1)   Ozone therapy improves red cell flexibility, enabling more oxygen transport.

2)   Ozone therapy stimulates your RBCs to generate a compound called 2,3 DGP. Interestingly, this molecule enables your RBCs to release their payload of oxygen into your tissues. Less 2,3 DGP and your RBCs might actually hold on to the payload leaving your tissues to become oxygen starved.

3)   Ozone therapy increases RBC ATP. The increased energy in the red cells enable newly created RBCs to become "super gifted" in the words of researcher Velio Bocci, MD of Italy.

4)   Ozone therapy appears to turn on overall mitochondrial function in your cells. Mitochondria are your cell furnaces where energy is made. More energy, more ability to repair, no matter the tissue or organ. This conclusion is based on basic science research of German and Cuban researchers showing more oxygen consumption in tissues.

5)   Researchers Bocci in Italy and Silvia Menendez, PhD and her team in Cuba have determined that ozone modulates the immune system. In other words, where there is inflammation that is not needed, ozone will dampen it, and allow inflamed tissues to heal. Where the immune system is weak, ozone therapy picks it up. The net effect of ozone therapy is to bring your immune system to a healthier set point of balance. You need inflammation to fight invaders and repair, but when inflammation is not turned off, the inflammation itself then becomes destructive to your tissues. In the case of joints, modulating inflammation with ozone can sometimes lead to instant results.

6)   Ozone, in vitro [lab cultures], instantly oxidizes reduced sulfhydryls to disulfides. While ozone itself lasts only microseconds in blood, the reaction of ozone with the blood lipids leads to the production of more stable but still highly reactive oxygen species (i.e. peroxides) which would react similarly, mimicing the pro-oxidant mechanism of immune system defense.

7)   Activation of the immune system: Ozone administered at a concentration of between 30 and 55 µg/cc causes the greatest increase in the production of interferon and the greatest output of tumor necrosis factor and interleukin-2. The production of interleukin-2 launches an entire cascade of subsequent immunological reactions.



Conclusion:

    I am not a fool, nor do I suffer fools lightly. There are scores of alternative medical scams out there on the Internet of which one must continually be wary. Most alternative medical scams can easily be detected, if you study the promotional wording, plus simply follow the money! These scams are characteristically short-lived, around only long enough to make a killing, then vanish.  Ozone therapy has been around since the 1800's and has only gained more approval by those clinicians that have actually worked with it. The trouble is, most of the ozone critics over the years have not once worked with it, but they just know in their heart out of pure intuition of how toxic it is from shear logic!  Ozone therapy has often been labeled as a quack procedure not backed by scientific evidence by the medical powers that be;  plus, the wide spread myth of the deadly nature of air emboli doesn't help ozone out any either. The successes of ozone has literally scared the medical power players into very deliberate suppressive strategies over the years and something tells me its not because they have the consumers' safety in mind. Malaysia appears to be the latest country that has banned all forms of ozone protocols out of ignorance. The true reality: ozone is dirt cheap to generate, unpatentable, and can only be used when generated in minutes near the patient--not a good recipe for corporate profits!

     Dr. Rowen and Robins organized an educational teaching expedition to Sierra Leone to fight the ebola epidemic in 2014. Both physicians were so sure of the value of ozone DIV in fighting the ebola virus from years of working with this modality in the USA that they thought they could make a huge difference in the African viral fight. What scammer in the world would ever do such a thing as an humanitarian gesture, yet alone exposing themselves to death? The crux of this story ends with the two only able to work in that country for days before the Minister of Health demanded their expulsion. They were there long enough to train a handful of doctors, treating a few patients with great success. Rowen was told later by reliable sources that the Zmapp and Vaccine companies found out about their ozone work and put pressure on WHO (World Health Organization) which in turn put pressure on the Sierra Leone Health Ministry to remove the two Physicians from the country. And so goes life and the profit motive!  If you study the biographies of Robins and Rowen, they have been crusaders all of their lives for more effective medical care. It would have been all so much easier for those two, if they had followed the company-line out of med school as their colleagues have by being simply becoming expensive legal pharmaceutical drug pushers. They would have been far more financially comfortable now, but that is not what motivates their psyche.  It is difficult to be a true believer in a condemned procedure when your beliefs contradict the health corporations and power centers. All legitimate alternative medical practitioners know this danger to their careers, yet something in their soul says: persist.  All I know is what I have seen and researched. I have seen first-hand, the value of ozone DIV. Hopefully, you may too.







     The Robins Protocol has proven safe and efficacious for Howard Robins MD and I would advise anyone to follow it very closely as I tried to do with a few exceptions I will discuss later:



     A Tomco Ozone Generator is used at a 55 mcg/ml setting for all the patients in the study.

     A Terumo 27 gauge winged infusion set (scalp vein set) and a Terumo 60cc syringe are used for administration.

     The largest superficial veins in the forearm or hand are used along with PICC  (peripherally inserted central catheter) lines at times.   PICC lines require pushing some sterile saline or water through first before the gas.  After pushing 5cc of oxygen/ozone gas through the infusion set to sterilize and prevent any air that might be in the tubing from entering the body, the needle is inserted into a vein.   The intravenous push is performed at a flow rate from 1cc per 5 -15 seconds depending on the size and resistance of the vein.  Most treatments last from 1 to 8 minutes depending upon the volume of gas delivered to a maximum of 12 minutes for 180 -240ml  treatments.  Smaller veins require a slower push than larger veins.  All adult patients are given 20cc at the first treatment increasing 10cc per treatment until 55cc are achieved. This volume is held until at least 10 to 12 treatments are completed. Additional volumes are given then in 10cc increments until an additional 60cc's are achieved.  In some cases a total of 180-240cc are given each treatment.  The amount given depends on vein tolerance, patient reaction to "kill-off",  and the presenting medical problem(s) being treated.  Infusion frequency is at a minimum of three treatments per week to a maximum of 12  (the Robins Fast-Trac method or RFT).  RFT can be performed at a maximum of 2 treatments per day  with a minimum three-hour window between treatments for no more than 6 days in a row or in any combination of consecutive or non-consecutive days.  One day a week with no therapy is necessary for the body to have an opportunity to clean out the waste created by the kill off from the treatments more completely. Volume, concentration and frequency is dependent upon:  body size with smaller people being fully perfused with less gas than larger people;  medical problem with more serious problems requiring more gas;  ability of the individual to tolerate the waste created and not develop Herksheimer/Jarrisch reactions following treatment;  lung reactions including bronchospasm or a feeling of tightness developing in the upper chest  (what this effect is will be discussed later).  Volume, concentration,  frequency and rate of administration are adjusted to each patient's individual needs and reactions as necessary at each treatment.  Over the years we have learned how to push the limits of concentration, volume, frequency and rate without causing undo vein damage or other adverse reactions.  Not a single patient has ever been harmed in anyway.   Every patient inducted into this protocol was fully informed of the risks and benefits and all consented to become part of our ongoing research studies. The Robins Method uses 27 gauge needles which makes it possible to treat almost any patient easily including children.  The use of this gauge needle also puts a stream of extremely small gas bubbles into the vein facilitating the safe dissolving of the gas in the blood and its attachment onto the red blood cells.

  We have used as much as 240cc's of oxygen/ozone gas, per therapy, without causing any adverse reaction of consequence.  Perhaps a larger amount might cause a problem but we have never offered more to any patient and  rarely use this amount.  Most get between 20cc and 115cc.




My Set-up and Method:
Ozone Generator . . . . . . .I would advise one to use only the best ozone generator you can afford. China of late has some interesting ozone medical generators very reasonably priced, but so far, I am suspect of their manufacturing codes and reliability.  My preference would be a western hemisphere product of which the Canadian manufactured Longevity Ext 120 currently is my choice--considering my economic limitations. It can produce the full concentration range, from below 1 ug/ml (considered 'micro-dosing') to one of the highest ozone concentrations in the world, 120 ug/ml.  It consists of a cold corona double-walled quartz glass tube, known as being capable of providing the purest ozone in the world because the ozone is never in contact with ceramic, metal, plastic, rubber, glues, resins, nor any other material that would otherwise contaminate the ozone. The current retail price for this unit is approximately US$2000-2500 from what I can see. One can often find used units on ebay much cheaper, but know that used units may be in need of calibration. Both Longevity and Promolife offer generator inspections on all brands which may be a useful service if buying used.  By the way, it was Longevity that donated their ozone units to be taken over to Sierra Leone by Dr. Robins and Rowen for their ebola work. They are made like tanks!  Most of the ozone medical specialists choose the much more pricey German generators such as Zotzmann and Herrmann. I think they go for around US$12,500.

USP Oxygen tank and regulator . . . . . . . . . doing ozone DIV, the use of USP grade oxygen is a must! Also, one would use a pediatric tank regulator capable of flow sequences of 1/32, 1/16, 1/8, 1/4, 1/2, 3/4, 1, 2, 3. It is often over-looked that regulators may be inaccurate! I would advise anyone to test their regulators before use. Flow rates are vital for accurate ozone concentrations! Testing is simple, simply run a hose connected to regulator into a bucket of water where the air displaces water in an inverted measuring device registering in ml units. Use a stop watch to see how much oxygen is released in a minute.

Syringe Infusion Pump . . . . . . . . . . . . .personally, I think this is an indispensable piece of instrumentation with ozone DIV.  Dr. Robins and others seem to disagree. I am currently using an old analog Harvard Model  975.  As I discussed previously, the old analog technology pumps are probably the safest to use. All pumps should also be periodically checked for accurate calibration with a ml volume cup and a stop watch. A good used one can sometimes be had quite reasonably with many different brand models on ebay.

The cardiac output is usually expressed in liters/minute. For someone weighing about 70 kg (154 lbs), the cardiac output at rest is about 5000 ml. /minute. In this case, if the heart rate is 70 beats/min, the stroke volume would be a little more than 70 ml/beat. Dr. Bocci writes that oxygen solubility at 98.6°F is only about 0.23 ml per 100 ml of plasmatic water and therefore venous plasma.  Lets use this as a guide to how fast we should inject ozone/oxygen into the blood stream safely. There will of course be variables and unique individual anomalies that could upset these suppositions of blood solubility, but it is at least a guideline based on general scientific evidence of a point we should not pass.  Thus, we know it takes 100 ml of blood to absorb 0.23  ml of oxygen & ozone. Therefore, a 154 pound resting man would pump approximately 5000 ml / minute pass that needle.  In theory at that rate, 11.5 ml of oxygen/ozone could be given per minute with the blood just being able to absorb it. Dr. Robins writes that human flow rate should be any where from 1cc per 5 -15 seconds. Using his fastest suggested flow rate of 1cc per 5 seconds, maximum flow rate would be 12cc per minute within my suggested spec calculation.

The cardiac output of a resting equine is said to be around 75ml/min per Kg of body weight.  Therefore, a 1000 pound horse would pump approximately 34,000 ml / minute pass that needle.  In theory, at that rate,  78.2 ml of oxygen/ozone could be given per minute with the blood just being able to absorb it.



Glass Syringes . . . . . . . . . . . . . I use two sizes, a 50cc and a 2cc syringe. I prefer glass, though one can use the popular plastic disposable models with equal reliability. I just feel more comfortable with the inert properties of glass to ozone plus, the glass models seem to have freer plunger action. The 50cc syringe is used for actual ozone/oxygen infusion into the vein while the small 2cc model is used in prepping the 27g butterfly vein set by pumping out the atmospheric air present with an ozone/oxygen mixture prior to use. The smaller 2cc syringe also acts as a stabilizing anchor to the butterfly set while it is being inserted into the patient's vein--which I find helpful. I do own a autoclave which is always a plus when employing reusable glass syringes.  Many do not.

Butterfly Scalp Vein Set . . . . . . . . . . . coming from the veterinary field, I was rather turned-off by the use of these types of needles. I was accustomed  to doing IVs on jugulars with 16-19g needles, an inch or more long. I soon found out in the case of humans, the 25-27g butterfly scalp vein needles were quite nice to use and relatively painless! They are superb for superficial veins common in humans and probably canines as well. Perhaps not so useful in the equine where the jugular lies much deeper, though saying that, I have acquired some 1.5" needles at 27g which I would like to experiment with on the equine and bovine jugulars in the future. I may be pleasantly surprised that they will work much better than I would anticipate. Certainly in theory, the smaller 27g gauge needle would be preferred for ozone/oxygen infusion as that small bore would product smaller bubbles in the vein, though I may be over-blowing the importance of this.

2-way stopcock on the ozone generator outlet tubing . . . . . . . . . . .  I consider this syringe fitting to be an important accessory to the generator. It allows the generator to continually maintain an ozone/oxygen gas flow until you actually fill the syringe when the lever can easily be switched over to direct the gas into the syringe.  For accurate ozone concentration out of the generator, one needs to warm-up the unit for 10-15 minutes prior to filling the syringe. With this 2-way stopcock, gas flow is never halted.

1-way stopcock on the syringe . . . . . . . . . . . . . though this syringe fitting is not necessary, I find it very useful as a safeguard that once the 50cc syringe is filled with the desired gas, the stopcock can easily be turned to seal the gas inside until it can be used.

Tourniquet . . . . . . . . . . . . . this is a very helpful accessory when finding and inserting the butterfly into the vein. The kind I have pictured is particularly useful when self-administering, one hand friendly. Of course in veterinary medicine, these are seldom used. I use deep finger pressure on the equine and bovine to distend the jugular.
Ozone DIV Cheat List:


1)   Warm up ozone generator 10+ minutes at ozone concentration desired to obtain a consistent output. On my Longevity Ext 120, I set it at a #3 setting at an oxygen tank flow of 1/16 liters per minute to get a 55 mcg concentration.

2)    Fill up small sterile syringe with ozone and pump through butterfly needle tubing displacing atmospheric air.

3)    Fill up large sterile syringe to 50cc and place on syringe pump, switch on, removing excess ozone amount in syringe to obtain desired amount to be infused. Switch off until ready to begin infusion.  This preps the pump mechanism for instantaneous delivery.

4)    Tourniquet upper arm, 3-6" from needle site. Apply antiseptic to site.

5)     Insert butterfly needle that has a small syringe attached which helps to stabilize tubing.

6)     When properly seated, one should see blood flash in butterfly  tube. Remove TOURNIQUET.

7)     Detach small syringe from butterfly and attach to ozone filled syringe on the pump.

8)     Switch syringe pump on monitoring needle site for proper placement.

NOTE:    Ozone is not a stable  structure and we need to be  able to manufacture it on site  for immediate use.  It typically has a half-life of  55 minutes in a 60cc disposable syringe.  Dr. Frank Shallenberger says ozone deterioration is even greater at 50% in 30 minutes in a plastic syringe. That is not a long time!  Ozone also is affected by temperature and air humidity. Ideally, fill syringe minutes before injection.
Ozone Administration in Horses
by Judith M. Shoemaker, DVM
My method of choice for systemic ozone administration in the horse is direct intravenous infusion of 70 ug/ml ozone in oxygen. Given at a reasonable rate (1-3 ml/sec), no significant side effects are usually encountered. I have administered thousands of ozone treatments in the last ten years with minimal reactions occurring only rarely. The most common reaction is an "itchy" nose if the administration rate is too fast. I usually listen to the heart the first time a horse is given ozone as the hyper-oxygenation of the heart will slow the rate significantly and heart rates below 18 bpm can lead to syncope. In athletic horses, one and two degree heart blocks are common and will be more frequent during ozone administration. I reduce the rate of administration if blocks occur more often than every four beats, as blood pressure drops occur with lowered rate and two-degree blocks.

The hyper-oxygenation of the gut can cause increased peristalsis and some horses show a very transient gas pain reaction. This usually occurs within 20 minutes of administration and lasts for, at most, 10-15 minutes. We usually give Rescue Remedy and Nux Vomica homeopathic and entertain them, and the discomfort resolves quickly. Severely toxic horses may have skin detoxification signs in a few days. Continued treatment creates phenomenal hair, mane, tail and hoof growth (up to 1⁄2" or more per month). All animals we have treated have benefited, and many have been "miracle cures".

I have treated cases of laminitis, septic arthritis and tenosynovitis, lymphosarcoma, endocarditis, myocarditis, C.O.P.D., allergies, colic, lymphangitis, abscesses, osteomyelitis, fistulas (poll and wither), candidiasis, periodic opthalmia, melanoma, Rocky Mountain Spotted Fever, Lyme disease, Babesiosis, West Nile, EPM, herpes myelitis, sinusitis, tooth abscesses, soft tissue infections, bladder and kidney infections and chronic liver and kidney disease all with successful outcomes. Many completely resolved very rapidly, even long standing cases that had been treated unsuccessfully with the best of conventional medicine.

The procedure is simple. Making the ozone with Genesis' SST machine at 1/8 lpm flow of oxygen produces 70 ug/ml concentration ozone. Fill six 60cc syringes with the gas. Do not let them sit around, as the ozone will disintegrate the rubber of the syringe. One can use glass or non-reactive syringes; I just replace mine frequently. A 25-gauge needle (so bubbles are small) inserted in the jugular with a 24 inch IV extension set taped with duct tape is all that's needed to administer the treatment.

Give 1-3 ml/sec. and "strip" the jugular periodically during the treatment as the gas can accumulate in the empty vein as you proceed. It is almost impossible to create an embolism in a horse with this treatment, even if the vein fills completely, as the ozone and oxygen dissolve and are absorbed very rapidly.

Most often horses will look very content during and after the treatment. They soon look forward to it - especially if the first treatment is given at the rate described above and not faster. If an animal says his nose is very itchy or he seems uncomfortable after four 60cc syringes, we may reduce the dose to four syringes for 1-2 treatments. They usually tolerate all six after two treatments.

I usually recommend 8 treatments at 1-3 day intervals. I have treated animals with as many as 300 treatments over months with no side effects. Severe cases can be treated daily, but much older horses may want 2-3 days between treatments.

Even a few treatments will benefit, making other treatments more effective and accelerating healing. I often give a single ozone treatment after an adjustment and acupuncture session as the effects of other modalities of therapy are enhanced and any muscle soreness from reorganization and remodeling from joint reorientation are resolved and healed more rapidly. Ozone's effects are enhanced by Procaine and pain control is profound in some cases. The concurrent use of anti-inflammatories is contraindicated but is usually not needed, especially with homeopathic and/or neutraceuticals. One can use Palosein (orgotein) with ozone therapies.


Discussion:  One can't knock success or years of experience, but its interesting that she uses a concentration of  70 ug/ml  in six 60cc syringes or a total of 360 mls of ozone per treatment for what I presume is a typical 1000 lb horse.   In the past, 70 ug/ml  would have been the upper reaches of acceptability.   Probably not so much any more with the lower concentration guidelines.  According to the Viebahn-Hansler article, Ozone in Medicine (2012),  the low dose concept (Hormesis)  has become the proven dosage platform of choice for ozone with any dosage over 69 ug/ml  considered in the toxic region. If that be the case, she is using a rather high concentration for the animal's needs and may be better off with a range around 20 ug/ml  with maximum  of 55 ug/ml which matches the article's MAH guidelines. Her total dosage volume is close to the recommended volume used in humans  for MAH.  She has a good idea about using a small gauge needle (25g) to reduce bubble size in the jugular along with a IV extension and monitoring the heart rate.  Any change in the heart rate should be carefully acknowledged and treatment rate adjusted.  Perhaps the commonly available equine heart rate monitors would be a very useful device to utilize when treating  a horse with ozone?   Lastly, it is interesting that she equated "nose itching" with a too  quick administration of ozone.  This might be a very useful tip and should be  monitored closely.  Lets look at her injection rate of 1-3ml per second (60-180ml  per minute).  According to my calculations above,  she may be injecting a bit too fast  since I feel anything over 78ml per minute in the equine blood stream may have a hard time with instant solubility.   Her slower rates are within that range, not her faster ones.  However, saying this, we do not live in a standardized world.  Her horses may not have  a cardiac output equal to a resting rate. As we know, vets can easily get horses' hearts racing resulting in increased blood flows able to take care of larger injection rates.  It all  depends.

Georgian Med News. 2006 Sep;(138):93-5.


Influence of intravenous ozone treatment on the level of different specificity antibodies
[Article in Russian]

Mandzhgaladze NR, Kharebava ER, Didia TsG, Ardzhevanishvili MD, Gudzhabidze MV, Chigiashvili TsN.

Abstract:

Medical ozone is the universal stimulator which participates in intracellular biochemical processes. Treatment with intravenous ozone was studied in 35 women, 20 of them with gestosis Rhesus sensibility, 3--with anti-HLA antibodies, 5--pregnant with ABO sensibility, 3--with anti-sperm antibodies, and 7- with antivirus antibodies (Herpes 1,2 and CMV). As a result, ozone treatment is effective for decrease anti-erythrocyte and anti-leukocyte antibodies and other antibody levels in blood. Medical ozone has direct antiviral activity which induces long term remission and in some cases total elimination of virus from blood. Generally, ozone is a modulator of the immune system, stimulating links of humoral and cell immunity. It appeared that index of immune regulation (T-helper/T suppressor) in pregnant women was increased and level of immunoglobulins (Ig G, M, A) was within normal ranges. This work shows the results of chemical influence of ozone on the antibodies which subsequently the decreases the level of modifying immunoglobulins.